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TitlePlant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease.
Journal, issue, pagesFront Plant Sci, Vol. 10, Page 880, Year 2019
Publish dateJul 9, 2019
AuthorsJohanna Marsian / Daniel L Hurdiss / Neil A Ranson / Anneli Ritala / Richard Paley / Irene Cano / George P Lomonossoff /
PubMed AbstractVirus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in plants. VLPs could also be ...Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass () showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.
External linksFront Plant Sci / PubMed:31354759 / PubMed Central
MethodsEM (single particle)
Resolution3.7 Å
Structure data

4899

6rj0

EMDB-4899, PDB-6rj0:
Atlantic Cod Nervous Necrosis Virus-Like Particle produced in Nicotiana benthamiana
Method: EM (single particle) / Resolution: 3.7 Å

Source
  • betanodavirus sp.
KeywordsVIRUS LIKE PARTICLE / Betanodavirus / Virus-like particle / Virus / Vaccine

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