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Structure paper

TitleStructures of N-terminally processed KRAS provide insight into the role of N-acetylation.
Journal, issue, pagesSci Rep, Vol. 9, Page 10512-10512, Year 2019
Publish dateAug 24, 2018 (structure data deposition date)
AuthorsDharmaiah, S. / Tran, T.H. / Messing, S. / Agamasu, C. / Gillette, W.K. / Yan, W. / Waybright, T. / Alexander, P. / Esposito, D. / Nissley, D.V. ...Dharmaiah, S. / Tran, T.H. / Messing, S. / Agamasu, C. / Gillette, W.K. / Yan, W. / Waybright, T. / Alexander, P. / Esposito, D. / Nissley, D.V. / McCormick, F. / Stephen, A.G. / Simanshu, D.K.
External linksSci Rep / PubMed:31324887
MethodsX-ray diffraction
Resolution1.011 - 1.5 Å
Structure data

PDB-6m9w:
Structure of Mg-free KRAS4b (2-169) bound to GDP with the switch-I in fully open conformation
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-6mbq:
Crystal structure of Mg-free wild-type KRAS (2-166) bound to GMPPNP in the state 1 conformation
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-6mbt:
Crystal structure of wild-type KRAS bound to GDP and Mg (Space group C2)
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-6mbu:
Crystal structure of wild-type KRAS (1-169) bound to GDP and Mg (Space group P3)
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-6p0z:
Crystal structure of N-acetylated KRAS (2-169) bound to GDP and Mg
Method: X-RAY DIFFRACTION / Resolution: 1.011 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-ACE:
ACETYL GROUP

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • homo sapiens (human)
KeywordsONCOPROTEIN / KRAS / RAS / Mg / K-RAS-4b / Small GTPase / Guanine nucleotide / K-RAS / GMPPNP / GppNHp / GDP

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