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Structure paper

TitleDistinct RNA-binding modules in a single PUF protein cooperate to determine RNA specificity.
Journal, issue, pagesNucleic Acids Res., Vol. 47, Page 8770-8784, Year 2019
Publish dateFeb 7, 2019 (structure data deposition date)
AuthorsQiu, C. / Dutcher, R.C. / Porter, D.F. / Arava, Y. / Wickens, M. / Hall, T.M.T.
External linksNucleic Acids Res. / PubMed:31294800
MethodsX-ray diffraction
Resolution1.554 - 3.002 Å
Structure data

PDB-6nww:
Crystal structure of the RRM domain of S. pombe Puf1 in the P212121 space group
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

PDB-6nx5:
Crystal structure of the RRM domain of S. pombe Puf1 in the P21 space group
Method: X-RAY DIFFRACTION / Resolution: 1.554 Å

PDB-6ny5:
Crystal structure of the PUM-HD domain of S. pombe Puf1 in complex with RNA
Method: X-RAY DIFFRACTION / Resolution: 3.002 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-SO4:
SULFATE ION

ChemComp-MG:
Unknown entry

Source
  • schizosaccharomyces pombe (fission yeast)
KeywordsRNA BINDING PROTEIN / RRM domain / the RRM domain / RNA BINDING PROTEIN/RNA / PUF protein / RNA BINDING PROTEIN-RNA complex

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