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TitleACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages.
Journal, issue, pagesPLoS Comput Biol, Vol. 15, Issue 7, Page e1007081, Year 2019
Publish dateJul 10, 2019
AuthorsChun Chan / Xiaoyun Pang / Yan Zhang / Tongxin Niu / Shengjiang Yang / Daohui Zhao / Jian Li / Lanyuan Lu / Victor W Hsu / Jian Zhou / Fei Sun / Jun Fan /
PubMed AbstractStudies on the Bin-Amphiphysin-Rvs (BAR) domain have advanced a fundamental understanding of how proteins deform membrane. We previously showed that a BAR domain in tandem with a Pleckstrin Homology ...Studies on the Bin-Amphiphysin-Rvs (BAR) domain have advanced a fundamental understanding of how proteins deform membrane. We previously showed that a BAR domain in tandem with a Pleckstrin Homology (PH domain) underlies the assembly of ACAP1 (Arfgap with Coil-coil, Ankryin repeat, and PH domain I) into an unusual lattice structure that also uncovers a new paradigm for how a BAR protein deforms membrane. Here, we initially pursued computation-based refinement of the ACAP1 lattice to identify its critical protein contacts. Simulation studies then revealed how ACAP1, which dimerizes into a symmetrical structure in solution, is recruited asymmetrically to the membrane through dynamic behavior. We also pursued electron microscopy (EM)-based structural studies, which shed further insight into the dynamic nature of the ACAP1 lattice assembly. As ACAP1 is an unconventional BAR protein, our findings broaden the understanding of the mechanistic spectrum by which proteins assemble into higher-ordered structures to achieve membrane deformation.
External linksPLoS Comput Biol / PubMed:31291238 / PubMed Central
MethodsEM (helical sym.)
Resolution14 Å
Structure data

PDB-5h3d:
Helical structure of membrane tubules decorated by ACAP1 (BARPH doamin) protein by cryo-electron microscopy and MD simulation
Method: ELECTRON MICROSCOPY / Resolution: 14.0 Å

Source
  • homo sapiens (human)
KeywordsSIGNALING PROTEIN / ACAP1 BARPH domain / membrane remodeling / molecular dynamics simulation

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