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TitleAn allosteric network in spastin couples multiple activities required for microtubule severing.
Journal, issue, pagesNat Struct Mol Biol, Vol. 26, Issue 8, Page 671-678, Year 2019
Publish dateJul 8, 2019
AuthorsColby R Sandate / Agnieszka Szyk / Elena A Zehr / Gabriel C Lander / Antonina Roll-Mecak /
PubMed AbstractThe AAA+ ATPase spastin remodels microtubule arrays through severing and its mutation is the most common cause of hereditary spastic paraplegias (HSP). Polyglutamylation of the tubulin C-terminal ...The AAA+ ATPase spastin remodels microtubule arrays through severing and its mutation is the most common cause of hereditary spastic paraplegias (HSP). Polyglutamylation of the tubulin C-terminal tail recruits spastin to microtubules and modulates severing activity. Here, we present a ~3.2 Å resolution cryo-EM structure of the Drosophila melanogaster spastin hexamer with a polyglutamate peptide bound in its central pore. Two electropositive loops arranged in a double-helical staircase coordinate the substrate sidechains. The structure reveals how concurrent nucleotide and substrate binding organizes the conserved spastin pore loops into an ordered network that is allosterically coupled to oligomerization, and suggests how tubulin tail engagement activates spastin for microtubule disassembly. This allosteric coupling may apply generally in organizing AAA+ protein translocases into their active conformations. We show that this allosteric network is essential for severing and is a hotspot for HSP mutations.
External linksNat Struct Mol Biol / PubMed:31285604 / PubMed Central
MethodsEM (single particle)
Resolution3.2 Å
Structure data

20226

6p07

EMDB-20226, PDB-6p07:
Spastin hexamer in complex with substrate
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • drosophila melanogaster (fruit fly)
KeywordsMOTOR PROTEIN / AAA+ ATPase / Homohexamer / Microtubule Severing Enzyme

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