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Structure paper

TitleSmoothened stimulation by membrane sterols drives Hedgehog pathway activity.
Journal, issue, pagesNature, Vol. 571, Page 284-288, Year 2019
Publish dateFeb 26, 2019 (structure data deposition date)
AuthorsDeshpande, I. / Liang, J. / Hedeen, D. / Roberts, K.J. / Zhang, Y. / Ha, B. / Latorraca, N.R. / Faust, B. / Dror, R.O. / Beachy, P.A. ...Deshpande, I. / Liang, J. / Hedeen, D. / Roberts, K.J. / Zhang, Y. / Ha, B. / Latorraca, N.R. / Faust, B. / Dror, R.O. / Beachy, P.A. / Myers, B.R. / Manglik, A.
External linksNature / PubMed:31263273
MethodsX-ray diffraction
Resolution2.8 Å
Structure data

PDB-6o3c:
Crystal structure of active Smoothened bound to SAG21k, cholesterol, and NbSmo8
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-NA:
Unknown entry

ChemComp-OLB:
(2S)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-LKD:
3-chloro-4,7-difluoro-N-{[2-methoxy-5-(pyridin-4-yl)phenyl]methyl}-N-[trans-4-(methylamino)cyclohexyl]-1-benzothiophene-2-carboxamide

ChemComp-CLR:
CHOLESTEROL

ChemComp-HOH:
WATER

Source
  • mus musculus (house mouse)
  • lama glama (llama)
KeywordsSIGNALING PROTEIN / GPCR / Signaling / Hedgehog / Smoothened / Cholesterol / SAG

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