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Structure paper

TitleStructural and biochemical characterization of 20 beta-hydroxysteroid dehydrogenase fromBifidobacterium adolescentisstrain L2-32.
Journal, issue, pagesJ. Biol. Chem., Vol. 294, Page 12040-12053, Year 2019
Publish dateAug 24, 2018 (structure data deposition date)
AuthorsDoden, H.L. / Pollet, R.M. / Mythen, S.M. / Wawrzak, Z. / Devendran, S. / Cann, I. / Koropatkin, N.M. / Ridlon, J.M.
External linksJ. Biol. Chem. / PubMed:31209107
MethodsX-ray diffraction
Resolution1.99 - 2.2 Å
Structure data

PDB-6m9u:
Structure of the apo-form of 20beta-Hydroxysteroid Dehydrogenase from Bifidobacterium adolescentis strain L2-32
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-6ow4:
Structure of the NADH-bound form of 20beta-Hydroxysteroid Dehydrogenase from Bifidobacterium adolescentis strain L2-32
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

Chemicals

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-CL:
Unknown entry

ChemComp-IMD:
IMIDAZOLE

ChemComp-HOH:
WATER

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

Source
  • bifidobacterium adolescentis l2-32 (bacteria)
KeywordsOXIDOREDUCTASE / pyridine nucleotide-dependent enzyme / short-chain dehydrogenase/reductase / NADH dependent

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