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TitleStructure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain.
Journal, issue, pagesStructure, Vol. 27, Issue 8, Page 1246-11257.e5, Year 2019
Publish dateAug 6, 2019
AuthorsKerstin K Viet / Annika Wagner / Kevin Schwickert / Nils Hellwig / Martha Brennich / Nicole Bader / Tanja Schirmeister / Nina Morgner / Hermann Schindelin / Ute A Hellmich /
PubMed AbstractTRPML2 is the least structurally characterized mammalian transient receptor potential mucolipin ion channel. The TRPML family hallmark is a large extracytosolic/lumenal domain (ELD) between ...TRPML2 is the least structurally characterized mammalian transient receptor potential mucolipin ion channel. The TRPML family hallmark is a large extracytosolic/lumenal domain (ELD) between transmembrane helices S1 and S2. We present crystal structures of the tetrameric human TRPML2 ELD at pH 6.5 (2.0 Å) and 4.5 (2.95 Å), corresponding to the pH values in recycling endosomes and lysosomes. Isothermal titration calorimetry shows Ca binding to the highly acidic central pre-pore loop which is abrogated at low pH, in line with a pH-dependent channel regulation model. Small angle X-ray scattering confirms the ELD dimensions in solution. Changes in pH or Ca concentration do not affect the protein's secondary structure, but can influence ELD oligomer integrity according to native mass spectrometry. Our data thus complete the set of high-resolution views of human TRPML channel ELDs and reveal some structural responses to the conditions the TRPML2 ELD encounters as the channel traffics through the endolysosomal system.
External linksStructure / PubMed:31178222
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution2 - 2.95 Å
Structure data

SASDEC7:
Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 7.4 without Calcium
Method: SAXS/SANS

SASDED7:
Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 7.4, 2mM Calcium
Method: SAXS/SANS

SASDEE7:
Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 6.5, 0.5 mM Calcium
Method: SAXS/SANS

SASDEF7:
Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 6.5 without Calcium
Method: SAXS/SANS

SASDEG7:
Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 4.5 without Calcium
Method: SAXS/SANS

SASDEH7:
Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 4.5, 0.5 mM Calcium
Method: SAXS/SANS

PDB-6hrr:
Structure of the TRPML2 ELD at pH 6.5
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-6hrs:
Structure of the TRPML2 ELD at pH 4.5
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / TRPML Channel Mucolipin Cation Channel Endolysosomal System

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