Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural snapshots of actively transcribing influenza polymerase.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 26, Issue 6, Page 460-470, Year 2019
Publish date	Jun 3, 2019
Authors	Tomas Kouba / Petra Drncová / Stephen Cusack /
PubMed Abstract	Influenza virus RNA-dependent RNA polymerase uses unique mechanisms to transcribe its single-stranded genomic viral RNA (vRNA) into messenger RNA. The polymerase is initially bound to a promoter ...Influenza virus RNA-dependent RNA polymerase uses unique mechanisms to transcribe its single-stranded genomic viral RNA (vRNA) into messenger RNA. The polymerase is initially bound to a promoter comprising the partially base-paired 3' and 5' extremities of the RNA. A short, capped primer, 'cap-snatched' from a nascent host polymerase II transcript, is directed towards the polymerase active site to initiate RNA synthesis. Here we present structural snapshots, as determined by X-ray crystallography and cryo-electron microscopy, of actively initiating influenza polymerase as it transitions towards processive elongation. Unexpected conformational changes unblock the active site cavity to allow establishment of a nine-base-pair template-product RNA duplex before the strands separate into distinct exit channels. Concomitantly, as the template translocates, the promoter base pairs are broken and the template entry region is remodeled. These structures reveal details of the influenza polymerase active site that will help optimize nucleoside analogs or other compounds that directly inhibit viral RNA synthesis.
External links	Nat Struct Mol Biol / PubMed:31160782 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	2.88 - 3.24 Å
Structure data	4511 6qcs EMDB-4511, PDB-6qcs: Influenza B polymerase pre-initiation complex Method: EM (single particle) / Resolution: 3.1 Å 4512 6qct EMDB-4512, PDB-6qct: Influenza B polymerase elongation complex Method: EM (single particle) / Resolution: 3.2 Å PDB-6qcv: Crystal structure of influenza B polymerase initiation state with capped 14-mer RNA primer and CTP Method: X-RAY DIFFRACTION / Resolution: 3.24 Å PDB-6qcw: Crystal structure of influenza B polymerase initiation state with capped 14-mer RNA primer Method: X-RAY DIFFRACTION / Resolution: 2.88 Å PDB-6qcx: Crystal structure of influenza B polymerase initiation state with capped 15-mer RNA primer Method: X-RAY DIFFRACTION / Resolution: 3.08 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-PO4: PHOSPHATE ION / Phosphate ChemComp-CTP: CYTIDINE-5'-TRIPHOSPHATE / Cytidine triphosphate ChemComp-POP: PYROPHOSPHATE 2- / Pyrophosphate
Source	influenza b virus influenza b virus (b/memphis/13/2003) synthetic construct (others)
Keywords	VIRAL PROTEIN / Influenza polzmerase / Influenza B Polymerase / RNA dependent RNA polymerase