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TitleStructural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9.
Journal, issue, pagesNat Commun, Vol. 10, Page 1968-1968, Year 2019
Publish dateMar 22, 2019 (structure data deposition date)
AuthorsHirano, S. / Abudayyeh, O.O. / Gootenberg, J.S. / Horii, T. / Ishitani, R. / Hatada, I. / Zhang, F. / Nishimasu, H. / Nureki, O.
External linksNat Commun / PubMed:31036811
MethodsX-ray diffraction
Resolution2.9 Å
Structure data

PDB-6joo:
Crystal structure of Corynebacterium diphtheriae Cas9 in complex with sgRNA and target DNA
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • corynebacterium diphtheriae (bacteria)
  • corynebacterium diphtheriae nctc 13129 (bacteria)
KeywordsHYDROLASE/DNA/RNA / CRISPR-Cas / Cas9 / PAM / DNA endonuclease / Ribonucleoprotein / HYDROLASE-DNA-RNA complex

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