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Structure paper

TitleThe crystal structure of dGTPase reveals the molecular basis of dGTP selectivity.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 116, Page 9333-9339, Year 2019
Publish dateApr 8, 2019 (structure data deposition date)
AuthorsBarnes, C.O. / Wu, Y. / Song, J. / Lin, G. / Baxter, E.L. / Brewster, A.S. / Nagarajan, V. / Holmes, A. / Soltis, S.M. / Sauter, N.K. ...Barnes, C.O. / Wu, Y. / Song, J. / Lin, G. / Baxter, E.L. / Brewster, A.S. / Nagarajan, V. / Holmes, A. / Soltis, S.M. / Sauter, N.K. / Ahn, J. / Cohen, A.E. / Calero, G.
External linksProc. Natl. Acad. Sci. USA / PubMed:31019074
MethodsX-ray diffraction
Resolution2.9 - 3.35 Å
Structure data

PDB-6oi7:
Se-Met structure of apo- Escherichia coli dGTPase
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-6oiv:
XFEL structure of Escherichia coli dGTPase
Method: X-RAY DIFFRACTION / Resolution: 3.06 Å

PDB-6oiw:
Structure of Escherichia coli dGTPase bound to dGTP-1-thiol
Method: X-RAY DIFFRACTION / Resolution: 3.35 Å

PDB-6oix:
Structure of Escherichia coli dGTPase bound to GTP
Method: X-RAY DIFFRACTION / Resolution: 3.15 Å

PDB-6oiy:
Structure of Escherichia coli bound to dGTP
Method: X-RAY DIFFRACTION / Resolution: 3.29 Å

Chemicals

ChemComp-MN:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-T8T:
2'-deoxyguanosine-5'-O-(1-thiotriphosphate)

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-DGT:
2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE

Source
  • escherichia coli (strain k12) (bacteria)
KeywordsMETAL BINDING PROTEIN / E.coli dGTPase / Metalloenzymes / dNTP Triphosphohydrolase / hydrolase / Metalloenzyme / XFEL / E. coli dGTPase

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