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Structure paper

TitleControl by Metals of Staphylopine Dehydrogenase Activity during Metallophore Biosynthesis.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 141, Page 5555-5562, Year 2019
Publish dateMay 29, 2018 (structure data deposition date)
AuthorsHajjar, C. / Fanelli, R. / Laffont, C. / Brutesco, C. / Cullia, G. / Tribout, M. / Nurizzo, D. / Borezee-Durant, E. / Voulhoux, R. / Pignol, D. ...Hajjar, C. / Fanelli, R. / Laffont, C. / Brutesco, C. / Cullia, G. / Tribout, M. / Nurizzo, D. / Borezee-Durant, E. / Voulhoux, R. / Pignol, D. / Lavergne, J. / Cavelier, F. / Arnoux, P.
External linksJ. Am. Chem. Soc. / PubMed:30901200
MethodsX-ray diffraction
Resolution2.05 - 2.3 Å
Structure data

PDB-6gmz:
Staphylopine dehydrogenase in complex with Histidine tag and citrate
Method: X-RAY DIFFRACTION / Resolution: 2.22 Å

PDB-6h31:
Staphylopine dehydrogenase in the apo state
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-6h3d:
Staphylopine dehydrogenase in complex with xNA
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-6h3f:
Staphylopine dehydrogenase in complex with staphylopine and NADP+
Method: X-RAY DIFFRACTION / Resolution: 2.21 Å

Chemicals

ChemComp-NH4:
AMMONIUM ION

ChemComp-CIT:
CITRIC ACID

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-2PE:
NONAETHYLENE GLYCOL / precipitant*YM

ChemComp-GOL:
GLYCEROL

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-DHI:
D-HISTIDINE

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-8UX:
(2~{S})-4-[[(2~{R})-3-(1~{H}-imidazol-4-yl)-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]-2-[[(2~{S})-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]butanoic acid

Source
  • staphylococcus aureus (bacteria)
  • staphylococcus aureus subsp. aureus n315 (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / staphylopine biosynthesis

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