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TitleStructural dynamics of bacteriophage P22 infection initiation revealed by cryo-electron tomography.
Journal, issue, pagesNat Microbiol, Vol. 4, Issue 6, Page 1049-1056, Year 2019
Publish dateMar 18, 2019
AuthorsChunyan Wang / Jiagang Tu / Jun Liu / Ian J Molineux /
PubMed AbstractFor successful infection, bacteriophages must overcome multiple barriers to transport their genome and proteins across the bacterial cell envelope. We use cryo-electron tomography to study the ...For successful infection, bacteriophages must overcome multiple barriers to transport their genome and proteins across the bacterial cell envelope. We use cryo-electron tomography to study the infection initiation of phage P22 in Salmonella enterica serovar Typhimurium, revealing how a channel forms to allow genome translocation into the cytoplasm. Our results show free phages that initially attach obliquely to the cell through interactions between the O antigen and two of the six tailspikes; the tail needle also abuts the cell surface. The virion then orients perpendicularly and the needle penetrates the outer membrane. The needle is released and the internal head protein gp7* is ejected and assembles into an extracellular channel that extends from the gp10 baseplate to the cell surface. A second protein, gp20, is ejected and assembles into a structure that extends the extracellular channel across the outer membrane into the periplasm. Insertion of the third ejected protein, gp16, into the cytoplasmic membrane probably completes the overall trans-envelope channel into the cytoplasm. Construction of a trans-envelope channel is an essential step during infection of Gram-negative bacteria by all short-tailed phages, because such virions cannot directly deliver their genome into the cell cytoplasm.
External linksNat Microbiol / PubMed:30886360 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution26.0 - 29.0 Å
Structure data

EMDB-9006:
Trans-envelope channel formed during initial infection of bacteriophage P22 in Salmonella
Method: EM (subtomogram averaging) / Resolution: 28.0 Å

EMDB-9007:
Channel structure formed by Gp16 defective mutant of bacteriophage P22 in Salmonella using cryoelectron tomography
Method: EM (subtomogram averaging) / Resolution: 29.0 Å

EMDB-9008:
Channel structure formed by Gp20-defective mutant of bacteriophage P22 in Salmonella using cryoelectron tomography
Method: EM (subtomogram averaging) / Resolution: 26.0 Å

EMDB-9009:
Channel structure formed by Gp7-defective mutant of bacteriophage P22 in Salmonella using cryoelectron tomography
Method: EM (subtomogram averaging) / Resolution: 28.0 Å

EMDB-9010:
Structure of bacteriophage P22 with trans-envelope channel in the Salmonella cell wall
Method: EM (subtomogram averaging) / Resolution: 29.0 Å

Source
  • Enterobacteria phage P22 (virus)

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