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Structure paper

TitleStructure of a bacterial ATP synthase.
Journal, issue, pagesElife, Vol. 8, Year 2019
Publish dateFeb 6, 2019
AuthorsHui Guo / Toshiharu Suzuki / John L Rubinstein /
PubMed AbstractATP synthases produce ATP from ADP and inorganic phosphate with energy from a transmembrane proton motive force. Bacterial ATP synthases have been studied extensively because they are the simplest ...ATP synthases produce ATP from ADP and inorganic phosphate with energy from a transmembrane proton motive force. Bacterial ATP synthases have been studied extensively because they are the simplest form of the enzyme and because of the relative ease of genetic manipulation of these complexes. We expressed the PS3 ATP synthase in , purified it, and imaged it by cryo-EM, allowing us to build atomic models of the complex in three rotational states. The position of subunit shows how it is able to inhibit ATP hydrolysis while allowing ATP synthesis. The architecture of the membrane region shows how the simple bacterial ATP synthase is able to perform the same core functions as the equivalent, but more complicated, mitochondrial complex. The structures reveal the path of transmembrane proton translocation and provide a model for understanding decades of biochemical analysis interrogating the roles of specific residues in the enzyme.
External linksElife / PubMed:30724163 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 4.8 Å
Structure data

9327

6n2d

EMDB-9327, PDB-6n2d:
Bacillus PS3 ATP synthase membrane region
Method: EM (single particle) / Resolution: 3.3 Å

9333

6n2y

EMDB-9333, PDB-6n2y:
Bacillus PS3 ATP synthase class 1
Method: EM (single particle) / Resolution: 3.0 Å

9334

6n2z

EMDB-9334, PDB-6n2z:
Bacillus PS3 ATP synthase class 2
Method: EM (single particle) / Resolution: 3.0 Å

9335

6n30

EMDB-9335, PDB-6n30:
Bacillus PS3 ATP synthase class 3
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-9336:
Bacillus PS3 ATP synthase FO region class 1
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-9337:
Bacillus PS3 ATP synthase FO region class 2
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-9338:
Bacillus PS3 ATP synthase FO region class 3
Method: EM (single particle) / Resolution: 4.8 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

Source
  • bacillus sp. ps3 (bacteria)
  • bacillus sp. (strain ps3) (bacteria)
KeywordsHYDROLASE / Bacterial ATP synthase

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