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TitleCryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 513, Year 2019
Publish dateJan 31, 2019
AuthorsYu-Hua Lo / Mack Sobhany / Allen L Hsu / Brittany L Ford / Juno M Krahn / Mario J Borgnia / Robin E Stanley /
PubMed AbstractRix7 is an essential type II AAA-ATPase required for the formation of the large ribosomal subunit. Rix7 has been proposed to utilize the power of ATP hydrolysis to drive the removal of assembly ...Rix7 is an essential type II AAA-ATPase required for the formation of the large ribosomal subunit. Rix7 has been proposed to utilize the power of ATP hydrolysis to drive the removal of assembly factors from pre-60S particles, but the mechanism of release is unknown. Rix7's mammalian homolog, NVL2 has been linked to cancer and mental illness disorders, highlighting the need to understand the molecular mechanisms of this essential machine. Here we report the cryo-EM reconstruction of the tandem AAA domains of Rix7 which form an asymmetric stacked homohexameric ring. We trapped Rix7 with a polypeptide in the central channel, revealing Rix7's role as a molecular unfoldase. The structure establishes that type II AAA-ATPases lacking the aromatic-hydrophobic motif within the first AAA domain can engage a substrate throughout the entire central channel. The structure also reveals that Rix7 contains unique post-α7 insertions within both AAA domains important for Rix7 function.
External linksNat Commun / PubMed:30705282 / PubMed Central
MethodsEM (single particle)
Resolution4.5 Å
Structure data

9063

6mat

EMDB-9063, PDB-6mat:
Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7
Method: EM (single particle) / Resolution: 4.5 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Source
  • chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719) (fungus)
  • chaetomium thermophilum var. thermophilum dsm 1495 (fungus)
KeywordsRIBOSOMAL PROTEIN / AAA-ATPase

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