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TitleCharacterization of two steroid hydroxylases from different Streptomyces spp. and their ligand-bound and -unbound crystal structures.
Journal, issue, pagesFebs J., Vol. 286, Page 1683-1699, Year 2019
Publish dateJul 3, 2018 (structure data deposition date)
AuthorsDangi, B. / Lee, C.W. / Kim, K.H. / Park, S.H. / Yu, E.J. / Jeong, C.S. / Park, H. / Lee, J.H. / Oh, T.J.
External linksFebs J. / PubMed:30552795
MethodsX-ray diffraction
Resolution2.19 - 2.71 Å
Structure data

PDB-6a7i:
CYP154C4 from Streptomyces sp. W2061
Method: X-RAY DIFFRACTION / Resolution: 2.19 Å

PDB-6a7j:
Testerone bound CYP154C4 from Streptomyces sp. ATCC 11861
Method: X-RAY DIFFRACTION / Resolution: 2.71 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-HOH:
WATER

ChemComp-TES:
TESTOSTERONE / hormone*YM

Source
  • streptomyces sp. js01 (bacteria)
  • streptomyces violaceoruber (bacteria)
KeywordsOXIDOREDUCTASE / cytochrome P450 / Streptomyces / steroid hydroxylase

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