Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Crystal Structure of Aldehyde Dehydrogenase 16 Reveals Trans-Hierarchical Structural Similarity and a New Dimer.
Journal, issue, pages	J Mol Biol, Vol. 431, Issue 3, Page 524-541, Year 2019
Publish date	Feb 1, 2019
Authors	Li-Kai Liu / John J Tanner /
PubMed Abstract	The aldehyde dehydrogenase (ALDH) superfamily is a vast group of enzymes that catalyze the NAD-dependent oxidation of aldehydes to carboxylic acids. ALDH16 is perhaps the most enigmatic member of the ...The aldehyde dehydrogenase (ALDH) superfamily is a vast group of enzymes that catalyze the NAD-dependent oxidation of aldehydes to carboxylic acids. ALDH16 is perhaps the most enigmatic member of the superfamily, owing to its extra C-terminal domain of unknown function and the absence of the essential catalytic cysteine residue in certain non-bacterial ALDH16 sequences. Herein we report the first production of recombinant ALDH16, the first biochemical characterization of ALDH16, and the first crystal structure of ALDH16. Recombinant expression systems were generated for the bacterial ALDH16 from Loktanella sp. and human ALDH16A1. Four high-resolution crystal structures of Loktanella ALDH16 were determined. Loktanella ALDH16 is found to be a bona fide enzyme, exhibiting NAD-binding, ALDH activity, and esterase activity. In contrast, human ALDH16A1 apparently lacks measurable aldehyde oxidation activity, suggesting that it is a pseudoenzyme, consistent with the absence of the catalytic Cys in its sequence. The fold of ALDH16 comprises three domains: NAD-binding, catalytic, and C-terminal. The latter is unique to ALDH16 and features a Rossmann fold connected to a protruding β-flap. The tertiary structural interactions of the C-terminal domain mimic the quaternary structural interactions of the classic ALDH superfamily dimer, a phenomenon we call "trans-hierarchical structural similarity." ALDH16 forms a unique dimer in solution, which mimics the classic ALDH superfamily dimer-of-dimer tetramer. Small-angle X-ray scattering shows that human ALDH16A1 has the same dimeric structure and fold as Loktanella ALDH16. We suggest that the Loktanella ALDH16 structure may be considered to be the archetype of the ALDH16 family.
External links	J Mol Biol / PubMed:30529746 / PubMed Central
Methods	SAS (X-ray synchrotron) / X-ray diffraction
Resolution	1.488 - 2.3 Å
Structure data	SASDE47: Aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16): 2 mg/ml Method: SAXS/SANS SASDE57: Aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16): 4 mg/ml Method: SAXS/SANS SASDE67: Aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16): 6 mg/ml Method: SAXS/SANS SASDE77: Aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16): 8 mg/ml Method: SAXS/SANS SASDE87: Aldehyde dehydrogenase family 16 member A1 from Homo sapiens (HsALDH16A1): 1 mg/ml Method: SAXS/SANS SASDE97: Aldehyde dehydrogenase family 16 member A1 from Homo sapiens (HsALDH16A1): 1.6 mg/ml Method: SAXS/SANS SASDEA7: Aldehyde dehydrogenase family 16 member A1 from Homo sapiens (HsALDH16A1): 3.2 mg/ml Method: SAXS/SANS PDB-6mvr: Structure of a bacterial ALDH16 Method: X-RAY DIFFRACTION / Resolution: 1.95 Å PDB-6mvs: Structure of a bacterial ALDH16 complexed with NAD Method: X-RAY DIFFRACTION / Resolution: 1.65 Å PDB-6mvt: Structure of a bacterial ALDH16 complexed with NADH Method: X-RAY DIFFRACTION / Resolution: 2.3 Å PDB-6mvu: Structure of a bacterial ALDH16 active site mutant C295A complexed with p-nitrophenylacetate Method: X-RAY DIFFRACTION / Resolution: 1.488 Å
Chemicals	ChemComp-SO4: SULFATE ION / Sulfate ChemComp-GOL: GLYCEROL / Glycerol ChemComp-HOH: WATER / Water ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM / Nicotinamide adenine dinucleotide ChemComp-NAI: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE / Nicotinamide adenine dinucleotide ChemComp-NA: Unknown entry ChemComp-K4V*: 4-nitrophenyl acetate
Source	loktanella sp. 3andimar09 (bacteria) Homo sapiens (human)
Keywords	OXIDOREDUCTASE / ALDEHYDE DEHYDROGENASE / ALDH16 / NAD / ROSSMANN FOLD