Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure and architecture of immature and mature murine leukemia virus capsids.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 115, Issue 50, Page E11751-E11760, Year 2018
Publish date	Dec 11, 2018
Authors	Kun Qu / Bärbel Glass / Michal Doležal / Florian K M Schur / Brice Murciano / Alan Rein / Michaela Rumlová / Tomáš Ruml / Hans-Georg Kräusslich / John A G Briggs /
PubMed Abstract	Retroviruses assemble and bud from infected cells in an immature form and require proteolytic maturation for infectivity. The CA (capsid) domains of the Gag polyproteins assemble a protein lattice as ...Retroviruses assemble and bud from infected cells in an immature form and require proteolytic maturation for infectivity. The CA (capsid) domains of the Gag polyproteins assemble a protein lattice as a truncated sphere in the immature virion. Proteolytic cleavage of Gag induces dramatic structural rearrangements; a subset of cleaved CA subsequently assembles into the mature core, whose architecture varies among retroviruses. Murine leukemia virus (MLV) is the prototypical γ-retrovirus and serves as the basis of retroviral vectors, but the structure of the MLV CA layer is unknown. Here we have combined X-ray crystallography with cryoelectron tomography to determine the structures of immature and mature MLV CA layers within authentic viral particles. This reveals the structural changes associated with maturation, and, by comparison with HIV-1, uncovers conserved and variable features. In contrast to HIV-1, most MLV CA is used for assembly of the mature core, which adopts variable, multilayered morphologies and does not form a closed structure. Unlike in HIV-1, there is similarity between protein-protein interfaces in the immature MLV CA layer and those in the mature CA layer, and structural maturation of MLV could be achieved through domain rotations that largely maintain hexameric interactions. Nevertheless, the dramatic architectural change on maturation indicates that extensive disassembly and reassembly are required for mature core growth. The core morphology suggests that wrapping of the genome in CA sheets may be sufficient to protect the MLV ribonucleoprotein during cell entry.
External links	Proc Natl Acad Sci U S A / PubMed:30478053 / PubMed Central
Methods	EM (subtomogram averaging) / EM (tomography) / X-ray diffraction
Resolution	1.89 - 8.6 Å
Structure data	0290 6hwi EMDB-0290, PDB-6hwi: Immature M-PMV capsid hexamer structure in intact virus particles Method: EM (subtomogram averaging) / Resolution: 7.2 Å 0291 6hww EMDB-0291, PDB-6hww: Immature MLV capsid hexamer structure in intact virus particles Method: EM (subtomogram averaging) / Resolution: 6.6 Å 0292 6hwx EMDB-0292, PDB-6hwx: Mature MLV capsid hexamer structure in intact virus particles Method: EM (subtomogram averaging) / Resolution: 7.2 Å 0293 6hwy EMDB-0293, PDB-6hwy: Mature MLV capsid pentamer structure in intact virus particles Method: EM (subtomogram averaging) / Resolution: 8.6 Å EMDB-4419: Representative tomogram of mature MLV particles Method: EM (tomography) EMDB-4421: Representative tomogram of immature M-PMV particles Method: EM (tomography) EMDB-4422: Representative tomogram of immature MLV particles Method: EM (tomography) PDB-6gza: Structure of murine leukemia virus capsid C-terminal domain Method: X-RAY DIFFRACTION / Resolution: 1.89 Å
Chemicals	ChemComp-CO: Unknown entry ChemComp-HOH: WATER / Water
Source	mason-pfizer monkey virus murine leukemia virus
Keywords	VIRAL PROTEIN / capsid / dimer / cobalt / M-PMV / hexamer / MLV / pentamer