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Structure paper

TitleMining the cellular inventory of pyridoxal phosphate-dependent enzymes with functionalized cofactor mimics.
Journal, issue, pagesNat Chem, Vol. 10, Page 1234-1245, Year 2018
Publish dateMar 29, 2018 (structure data deposition date)
AuthorsHoegl, A. / Nodwell, M.B. / Kirsch, V.C. / Bach, N.C. / Pfanzelt, M. / Stahl, M. / Schneider, S. / Sieber, S.A.
External linksNat Chem / PubMed:30297752
MethodsX-ray diffraction
Resolution1.9 - 2.45 Å
Structure data

PDB-6g56:
Apo-structure of the alanine racemase from Staphylococcus aureus
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-6g58:
Structure of the alanine racemase from Staphylococcus aureus in complex with a pyridoxal 5' phosphate-derivative
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6g59:
Structure of the alanine racemase from Staphylococcus aureus in complex with an pyridoxal-6- phosphate derivative
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-EOW:
(6-but-3-ynyl-4-methyl-5-oxidanyl-pyridin-3-yl)methyl dihydrogen phosphate

ChemComp-NA:
Unknown entry

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-ACT:
ACETATE ION

ChemComp-CL:
Unknown entry

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL / precipitant*YM

ChemComp-EM2:
(6-ethynyl-4-methanoyl-5-oxidanyl-pyridin-3-yl)methyl dihydrogen phosphate

Source
  • staphylococcus aureus (strain mu50 / atcc 700699) (bacteria)
  • staphylococcus aureus (bacteria)
  • staphylococcus aureus subsp. aureus mu50 (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / pyridoxal 5 phosphate dependent / D-alanine biosynthesis

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