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Structure paper

TitleEngineered Biosynthesis of beta-Alkyl Tryptophan Analogues.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 57, Page 14764-14768, Year 2018
Publish dateMar 26, 2018 (structure data deposition date)
AuthorsBoville, C.E. / Scheele, R.A. / Koch, P. / Brinkmann-Chen, S. / Buller, A.R. / Arnold, F.H.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:30215880
MethodsX-ray diffraction
Resolution1.75 - 2.26 Å
Structure data

PDB-6cut:
Engineered Holo TrpB from Pyrococcus furiosus, PfTrpB7E6 with (2S,3S)-isopropylserine bound as the external aldimine
Method: X-RAY DIFFRACTION / Resolution: 1.77 Å

PDB-6cuv:
Engineered Holo TrpB from Pyrococcus furiosus, PfTrpB7E6
Method: X-RAY DIFFRACTION / Resolution: 2.26 Å

PDB-6cuz:
Engineered TrpB from Pyrococcus furiosus, PfTrpB7E6 with (2S,3R)-ethylserine bound as the amino-acrylate
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

Chemicals

ChemComp-FEJ:
(2S,3S)-3-hydroxy-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]-4-methylpentanoic

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-PO4:
PHOSPHATE ION

ChemComp-FEV:
(2E)-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]pent-2-enoic acid

Source
  • pyrococcus furiosus (archaea)
  • pyrococcus furiosus (strain atcc 43587 / dsm 3638 / jcm 8422 / vc1) (archaea)
KeywordsLYASE / external aldimine / (2S / 3S)-isopropylserine / internal aldimine / amino-acrylate / 3R)-ethylserine

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