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Structure paper

TitleInsight into GFPmut2 pH Dependence by Single Crystal Microspectrophotometry and X-ray Crystallography.
Journal, issue, pagesJ. Phys. Chem. B, Vol. 122, Page 11326-11337, Year 2018
Publish dateJun 1, 2018 (structure data deposition date)
AuthorsLolli, G. / Raboni, S. / Pasqualetto, E. / Benoni, R. / Campanini, B. / Ronda, L. / Mozzarelli, A. / Bettati, S. / Battistutta, R.
External linksJ. Phys. Chem. B / PubMed:30179482
MethodsX-ray diffraction
Resolution1.648 - 2.4 Å
Structure data

PDB-6go8:
Structure of GFPmut2 crystallized at pH 6
Method: X-RAY DIFFRACTION / Resolution: 1.648 Å

PDB-6go9:
Structure of GFPmut2 crystallized at pH 6 and transferred to pH 7
Method: X-RAY DIFFRACTION / Resolution: 1.672 Å

PDB-6gqg:
Structure of GFPmut2 crystallized at pH 8.5
Method: X-RAY DIFFRACTION / Resolution: 1.792 Å

PDB-6gqh:
Structure of GFPmut2 crystallized at pH 8.5 and transferred to pH 6
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-6grm:
Structure of GFPmut2 crystallized at pH 6 and transferred to pH 9
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-CA:
Unknown entry

Source
  • aequorea victoria (jellyfish)
KeywordsFLUORESCENT PROTEIN / beta barrel / bioluminescence

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