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TitleMachining protein microcrystals for structure determination by electron diffraction.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 115, Issue 38, Page 9569-9573, Year 2018
Publish dateSep 18, 2018
AuthorsHelen M E Duyvesteyn / Abhay Kotecha / Helen M Ginn / Corey W Hecksel / Emma V Beale / Felix de Haas / Gwyndaf Evans / Peijun Zhang / Wah Chiu / David I Stuart /
PubMed AbstractWe demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure ...We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffraction and micron scale of synchrotron microfocus beamlines. The demonstration that atomic information can be retained suggests that milling could provide such detail on sections cut from vitrified cells.
External linksProc Natl Acad Sci U S A / PubMed:30171169 / PubMed Central
MethodsEM (electron crystallography)
Resolution1.9 Å
Structure data

PDB-6h3b:
Lysozyme: Machining protein microcrystals for structure determination by electron diffraction
Method: ELECTRON CRYSTALLOGRAPHY / Resolution: 1.9 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

Source
  • gallus gallus (chicken)
KeywordsHYDROLASE / lysozyme

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