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TitleLigand discrimination and gating in cyclic nucleotide-gated ion channels from apo and partial agonist-bound cryo-EM structures.
Journal, issue, pagesElife, Vol. 7, Year 2018
Publish dateJul 20, 2018
AuthorsJan Rheinberger / Xiaolong Gao / Philipp Am Schmidpeter / Crina M Nimigean /
PubMed AbstractCyclic nucleotide-modulated channels have important roles in visual signal transduction and pacemaking. Binding of cyclic nucleotides (cAMP/cGMP) elicits diverse functional responses in different ...Cyclic nucleotide-modulated channels have important roles in visual signal transduction and pacemaking. Binding of cyclic nucleotides (cAMP/cGMP) elicits diverse functional responses in different channels within the family despite their high sequence and structure homology. The molecular mechanisms responsible for ligand discrimination and gating are unknown due to lack of correspondence between structural information and functional states. Using single particle cryo-electron microscopy and single-channel recording, we assigned functional states to high-resolution structures of SthK, a prokaryotic cyclic nucleotide-gated channel. The structures for apo, cAMP-bound, and cGMP-bound SthK in lipid nanodiscs, correspond to no, moderate, and low single-channel activity, respectively, consistent with the observation that all structures are in resting, closed states. The similarity between apo and ligand-bound structures indicates that ligand-binding domains are strongly coupled to pore and SthK gates in an allosteric, concerted fashion. The different orientations of cAMP and cGMP in the 'resting' and 'activated' structures suggest a mechanism for ligand discrimination.
External linksElife / PubMed:30028291 / PubMed Central
MethodsEM (single particle)
Resolution3.35 - 3.46 Å
Structure data

7482

6cjq

EMDB-7482, PDB-6cjq:
Structure of the SthK cyclic nucleotide-gated potassium channel
Method: EM (single particle) / Resolution: 3.42 Å

7483

6cjt

EMDB-7483, PDB-6cjt:
Structure of the SthK cyclic nucleotide-gated potassium channel in complex with cGMP
Method: EM (single particle) / Resolution: 3.46 Å

7484

6cju

EMDB-7484, PDB-6cju:
Structure of the SthK cyclic nucleotide-gated potassium channel in complex with cAMP
Method: EM (single particle) / Resolution: 3.35 Å

Chemicals

ChemComp-PGW:
(1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl / phospholipid*YM / Phosphatidylglycerol

ChemComp-PCG:
CYCLIC GUANOSINE MONOPHOSPHATE / Cyclic guanosine monophosphate

ChemComp-CMP:
ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE / Cyclic adenosine monophosphate

Source
  • Spirochaeta thermophila DSM 6578 (bacteria)
  • spirochaeta thermophila (bacteria)
KeywordsTRANSPORT PROTEIN / ion channel / tetramer / lipid / cyclic nucleotide / cGMP / cAMP

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