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TitleMechanism of premature translation termination on a sense codon.
Journal, issue, pagesJ Biol Chem, Vol. 293, Issue 32, Page 12472-12479, Year 2018
Publish dateAug 10, 2018
AuthorsEgor Svidritskiy / Gabriel Demo / Andrei A Korostelev /
PubMed AbstractAccurate translation termination by release factors (RFs) is critical for the integrity of cellular proteomes. Premature termination on sense codons, for example, results in truncated proteins, whose ...Accurate translation termination by release factors (RFs) is critical for the integrity of cellular proteomes. Premature termination on sense codons, for example, results in truncated proteins, whose accumulation could be detrimental to the cell. Nevertheless, some sense codons are prone to triggering premature termination, but the structural basis for this is unclear. To investigate premature termination, we determined a cryo-EM structure of the 70S ribosome bound with RF1 in response to a UAU (Tyr) sense codon. The structure reveals that RF1 recognizes a UAU codon similarly to a UAG stop codon, suggesting that sense codons induce premature termination because they structurally mimic a stop codon. Hydrophobic interaction between the nucleobase of U3 (the third position of the UAU codon) and conserved Ile-196 in RF1 is important for misreading the UAU codon. Analyses of RNA binding in ribonucleoprotein complexes or by amino acids reveal that Ile-U packing is a frequent protein-RNA-binding motif with key functional implications. We discuss parallels with eukaryotic translation termination by the release factor eRF1.
External linksJ Biol Chem / PubMed:29941456 / PubMed Central
MethodsEM (single particle)
Resolution3.7 Å
Structure data

7970

6dnc

EMDB-7970, PDB-6dnc:
E.coli RF1 bound to E.coli 70S ribosome in response to UAU sense A-site codon
Method: EM (single particle) / Resolution: 3.7 Å

Source
  • escherichia coli (E. coli)
  • synthetic construct (others)
KeywordsRIBOSOME / RF1 / sense codon / cryo-EM

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