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Structure paper

TitleCrystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with a fluorescent probe.
Journal, issue, pagesBiochem. Biophys. Res. Commun., Vol. 500, Page 698-703, Year 2018
Publish dateApr 11, 2018 (structure data deposition date)
AuthorsRossi, F. / Morrone, C. / Massarotti, A. / Ferraris, D.M. / Valenti, A. / Perugino, G. / Miggiano, R.
External linksBiochem. Biophys. Res. Commun. / PubMed:29684348
MethodsX-ray diffraction
Resolution2 Å
Structure data

PDB-6ga0:
Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with SNAP-Vista Green
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-ETW:
5-[(4-methylphenyl)methylcarbamoyl]-2-(6-oxidanyl-3-oxidanylidene-4~{H}-xanthen-9-yl)benzoic acid

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

Source
  • sulfolobus solfataricus p2 (archaea)
KeywordsTRANSFERASE / O6-alkylguanine-DNA alkyltransferase / protein engineering / self-labeling protein tag / fluorescent probe / extremophiles

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