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Structure paper

TitleOn the (un)coupling of the chromophore, tongue interactions, and overall conformation in a bacterial phytochrome.
Journal, issue, pagesJ. Biol. Chem., Vol. 293, Page 8161-8172, Year 2018
Publish dateMar 16, 2017 (structure data deposition date)
AuthorsTakala, H. / Lehtivuori, H.K. / Berntsson, O. / Hughes, A. / Nanekar, R. / Niebling, S. / Panman, M. / Henry, L. / Menzel, A. / Westenhoff, S. / Ihalainen, J.A.
External linksJ. Biol. Chem. / PubMed:29622676
MethodsX-ray diffraction
Resolution1.34 - 3.6 Å
Structure data

PDB-5nfx:
Deinococcus radiodurans BphP PAS-GAF Y263F mutant
Method: X-RAY DIFFRACTION / Resolution: 1.34 Å

PDB-5nm3:
Deinococcus radiodurans BphP PAS-GAF-PHY Y263F mutant, pre-illuminated
Method: X-RAY DIFFRACTION / Resolution: 3.3 Å

PDB-5nwn:
Deinococcus radiodurans BphP PAS-GAF-PHY Y263F mutant, dark
Method: X-RAY DIFFRACTION / Resolution: 3.6 Å

Chemicals

ChemComp-ACT:
ACETATE ION

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-LBV:
3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium

ChemComp-HOH:
WATER

Source
  • deinococcus radiodurans r1 (radioresistant)
  • deinococcus radiodurans (strain atcc 13939 / dsm 20539 / jcm 16871 / lmg 4051 / nbrc 15346 / ncimb 9279 / r1 / vkm b-1422) (radioresistant)
KeywordsTRANSFERASE / Kinase / Photosensor / Phytochrome

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