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-Structure paper
| タイトル | A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1). |
|---|---|
| ジャーナル・号・ページ | Mol Biol Cell, Vol. 29, Issue 9, Page 1060-1074, Year 2018 |
| 掲載日 | 2018年5月1日 |
 著者 | Tomohiro Kubo / Yuqing Hou / Deborah A Cochran / George B Witman / Toshiyuki Oda /   |
| PubMed 要旨 | Motility of cilia/flagella is generated by a coordinated activity of thousands of dyneins. Inner dynein arms (IDAs) are particularly important for the formation of ciliary/flagellar waveforms, but ...Motility of cilia/flagella is generated by a coordinated activity of thousands of dyneins. Inner dynein arms (IDAs) are particularly important for the formation of ciliary/flagellar waveforms, but the molecular mechanism of IDA regulation is poorly understood. Here we show using cryoelectron tomography and biochemical analyses of Chlamydomonas flagella that a conserved protein FAP44 forms a complex that tethers IDA f (I1 dynein) head domains to the A-tubule of the axonemal outer doublet microtubule. In wild-type flagella, IDA f showed little nucleotide-dependent movement except for a tilt in the f β head perpendicular to the microtubule-sliding direction. In the absence of the tether complex, however, addition of ATP and vanadate caused a large conformational change in the IDA f head domains, suggesting that the movement of IDA f is mechanically restricted by the tether complex. Motility defects in flagella missing the tether demonstrates the importance of the IDA f-tether interaction in the regulation of ciliary/flagellar beating. |
 リンク | Mol Biol Cell / PubMed:29540525 / PubMed Central |
| 手法 | EM (サブトモグラム平均) |
| 解像度 | 40.0 - 60.0 Å |
| 構造データ |  EMDB-6866:  EMDB-6867:  EMDB-6868:  EMDB-6869:  EMDB-6870:  EMDB-6871:  EMDB-6872:  EMDB-6873:  EMDB-6874: |
| 由来 |
|
Chlamydomonas reinhardtii (クラミドモナス)