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Structure paper

TitleStructure of the nuclear exosome captured on a maturing preribosome.
Journal, issue, pagesScience, Vol. 360, Issue 6385, Page 219-222, Year 2018
Publish dateApr 13, 2018
AuthorsJan Michael Schuller / Sebastian Falk / Lisa Fromm / Ed Hurt / Elena Conti /
PubMed AbstractThe RNA exosome complex processes and degrades a wide range of transcripts, including ribosomal RNAs (rRNAs). We used cryo-electron microscopy to visualize the yeast nuclear exosome holocomplex ...The RNA exosome complex processes and degrades a wide range of transcripts, including ribosomal RNAs (rRNAs). We used cryo-electron microscopy to visualize the yeast nuclear exosome holocomplex captured on a precursor large ribosomal subunit (pre-60) during 7-to-5.8 rRNA processing. The cofactors of the nuclear exosome are sandwiched between the ribonuclease core complex (Exo-10) and the remodeled "foot" structure of the pre-60 particle, which harbors the 5.8 rRNA precursor. The exosome-associated helicase Mtr4 recognizes the preribosomal substrate by docking to specific sites on the 25 rRNA, captures the 3' extension of the 5.8 rRNA, and channels it toward Exo-10. The structure elucidates how the exosome forms a structural and functional unit together with its massive pre-60 substrate to process rRNA during ribosome maturation.
External linksScience / PubMed:29519915
MethodsEM (single particle)
Resolution3.9 - 4.6 Å
Structure data

4301

6fsz

EMDB-4301, PDB-6fsz:
Structure of the nuclear RNA exosome
Method: EM (single particle) / Resolution: 4.6 Å

4302

6ft6

EMDB-4302, PDB-6ft6:
Structure of the Nop53 pre-60S particle bound to the exosome nuclear cofactors
Method: EM (single particle) / Resolution: 3.9 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • Baker's yeast (brewer's yeast)
KeywordsRNA / RNA exosome / Ribosome / pre-ribosome / Mtr4 / Helicase

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