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TitleExpanding RNA binding specificity and affinity of engineered PUF domains.
Journal, issue, pagesNucleic Acids Res., Vol. 46, Page 4771-4782, Year 2018
Publish dateOct 14, 2017 (structure data deposition date)
AuthorsZhao, Y.Y. / Mao, M.W. / Zhang, W.J. / Wang, J. / Li, H.T. / Yang, Y. / Wang, Z. / Wu, J.W.
External linksNucleic Acids Res. / PubMed:29490074
MethodsX-ray diffraction
Resolution2.25 - 2.457 Å
Structure data

PDB-5ykh:
Crystal structure of the engineered nine-repeat PUF domain
Method: X-RAY DIFFRACTION / Resolution: 2.457 Å

PDB-5yki:
Crystal structure of the engineered nine-repeat PUF domain in complex with cognate 9nt-RNA
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsRNA BINDING PROTEIN / PUF repeats / engineered protein / RNA recognition / RNA BINDING PROTEIN/RNA / RNA BINDING PROTEIN-RNA complex

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