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Structure paper

TitleThermal stabilization of the deglycating enzyme Amadoriase I by rational design.
Journal, issue, pagesSci Rep, Vol. 8, Page 3042-3042, Year 2018
Publish dateJun 29, 2017 (structure data deposition date)
AuthorsRigoldi, F. / Donini, S. / Giacomina, F. / Sorana, F. / Redaelli, A. / Bandiera, T. / Parisini, E. / Gautieri, A.
External linksSci Rep / PubMed:29445091
MethodsX-ray diffraction
Resolution2.153 - 2.85 Å
Structure data

PDB-5oc2:
Crystal structure of Asp295Cys/Lys303Cys Amadoriase I mutant from Aspergillus Fumigatus
Method: X-RAY DIFFRACTION / Resolution: 2.85 Å

PDB-5oc3:
Crystal structure of Ser67Cys/Pro121Cys Amadoriase I mutant from Aspergillus Fumigatus
Method: X-RAY DIFFRACTION / Resolution: 2.153 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

Source
  • neosartorya fumigata (mold)
KeywordsOXIDOREDUCTASE / thermoresistance / flavin dependant enzyme / glycated aminoacid

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