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-Structure paper
タイトル | Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion. |
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ジャーナル・号・ページ | J Struct Biol, Vol. 202, Issue 3, Page 200-209, Year 2018 |
掲載日 | 2018年2月3日 |
著者 | Jose-Jesus Fernandez / Sam Li / Tanmay A M Bharat / David A Agard / |
PubMed 要旨 | Recent evidence suggests that the beam-induced motion of the sample during tilt-series acquisition is a major resolution-limiting factor in electron cryo-tomography (cryoET). It causes suboptimal ...Recent evidence suggests that the beam-induced motion of the sample during tilt-series acquisition is a major resolution-limiting factor in electron cryo-tomography (cryoET). It causes suboptimal tilt-series alignment and thus deterioration of the reconstruction quality. Here we present a novel approach to tilt-series alignment and tomographic reconstruction that considers the beam-induced sample motion through the tilt-series. It extends the standard fiducial-based alignment approach in cryoET by introducing quadratic polynomials to model the sample motion. The model can be used during reconstruction to yield a motion-compensated tomogram. We evaluated our method on various datasets with different sample sizes. The results demonstrate that our method could be a useful tool to improve the quality of tomograms and the resolution in cryoET. |
リンク | J Struct Biol / PubMed:29410148 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 9.0 Å |
構造データ | EMDB-12531: |
由来 |
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