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TitleDirected Evolution of Alcohol Dehydrogenase for Improved Stereoselective Redox Transformations of 1-Phenylethane-1,2-diol and Its Corresponding Acyloin.
Journal, issue, pagesBiochemistry, Vol. 57, Page 1059-1062, Year 2018
Publish dateJan 9, 2018 (structure data deposition date)
AuthorsHamnevik, E. / Maurer, D. / Enugala, T.R. / Chu, T. / Lofgren, R. / Dobritzsch, D. / Widersten, M.
External linksBiochemistry / PubMed:29384657
MethodsX-ray diffraction
Resolution1.71 - 2.5 Å
Structure data

PDB-6ffx:
Crystal structure of R. ruber ADH-A, mutant F43H
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-6ffz:
Crystal structure of R. ruber ADH-A, mutant F43H, Y54L
Method: X-RAY DIFFRACTION / Resolution: 1.71 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-HOH:
WATER

Source
  • rhodococcus sp. m8 (bacteria)
KeywordsOXIDOREDUCTASE / alcohol dehydrogenase mutant variant / NADH-dependent / Zn2+-dependent / Rossmann fold

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