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Structure paper

TitleEpsin and Sla2 form assemblies through phospholipid interfaces.
Journal, issue, pagesNat Commun, Vol. 9, Page 328-328, Year 2018
Publish dateAug 3, 2017 (structure data deposition date)
AuthorsGarcia-Alai, M.M. / Heidemann, J. / Skruzny, M. / Gieras, A. / Mertens, H.D.T. / Svergun, D.I. / Kaksonen, M. / Uetrecht, C. / Meijers, R.
External linksNat Commun / PubMed:29362354
MethodsX-ray diffraction
Resolution1.84 - 3.35 Å
Structure data

PDB-5on7:
The ENTH domain from epsin-2 in complex with phosphatidylinositol 4,5-bisphosphate (PIP2)
Method: X-RAY DIFFRACTION / Resolution: 3.35 Å

PDB-5onf:
The ENTH domain from epsin-1
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-5oo7:
The ENTH domain from epsin-2 in complex with phosphatidylinositol 4,5-bisphosphate (PIP2)
Method: X-RAY DIFFRACTION / Resolution: 1.84 Å

PDB-6enr:
The ENTH domain from epsin Ent2
Method: X-RAY DIFFRACTION / Resolution: 1.84 Å

Chemicals

ChemComp-PIO:
[(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate

ChemComp-SO4:
SULFATE ION

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719) (fungus)
KeywordsENDOCYTOSIS / Phospholipid binding / adaptor protein complex

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