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Structure paper

TitleAffimer proteins inhibit immune complex binding to Fc gamma RIIIa with high specificity through competitive and allosteric modes of action.
Journal, issue, pagesProc. Natl. Acad. Sci. U.S.A., Vol. 115, Page E72-E81, Year 2018
Publish dateDec 6, 2016 (structure data deposition date)
AuthorsRobinson, J.I. / Baxter, E.W. / Owen, R.L. / Thomsen, M. / Tomlinson, D.C. / Waterhouse, M.P. / Win, S.J. / Nettleship, J.E. / Tiede, C. / Foster, R.J. ...Robinson, J.I. / Baxter, E.W. / Owen, R.L. / Thomsen, M. / Tomlinson, D.C. / Waterhouse, M.P. / Win, S.J. / Nettleship, J.E. / Tiede, C. / Foster, R.J. / Owens, R.J. / Fishwick, C.W.G. / Harris, S.A. / Goldman, A. / McPherson, M.J. / Morgan, A.W.
External linksProc. Natl. Acad. Sci. U.S.A. / PubMed:29247053
MethodsX-ray diffraction
Resolution2.35 Å
Structure data

PDB-5ml9:
Cocrystal structure of Fc gamma receptor IIIa interacting with Affimer F4, a specific binding protein which blocks IgG binding to the receptor.
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-5mn2:
Cocrystal structure of Fc gamma receptor IIIa interacting with Affimer G3, a specific binding protein which blocks IgG binding to the receptor.
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-SO4:
SULFATE ION

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsIMMUNE SYSTEM / Fc gamma receptor IIIa Affimer Competitive inhibitor FCGR3A / Fc gamma receptor IIIa Affimer Allosteric inhibitor FCGR3A

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