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Structure paper

TitleCrystal structures of ternary complexes of archaeal B-family DNA polymerases.
Journal, issue, pagesPLoS ONE, Vol. 12, Page e0188005-e0188005, Year 2017
Publish dateJul 31, 2017 (structure data deposition date)
AuthorsKropp, H.M. / Betz, K. / Wirth, J. / Diederichs, K. / Marx, A.
External linksPLoS ONE / PubMed:29211756
MethodsX-ray diffraction
Resolution2.003 - 2.199 Å
Structure data

PDB-5omf:
Closed, ternary structure of KOD DNA polymerase
Method: X-RAY DIFFRACTION / Resolution: 2.092 Å

PDB-5omq:
Ternary complex of 9N DNA polymerase in the replicative state with three metal ions in the active site
Method: X-RAY DIFFRACTION / Resolution: 2.199 Å

PDB-5omv:
Ternary complex of 9N DNA polymerase in the replicative state with two metal ions in the active site
Method: X-RAY DIFFRACTION / Resolution: 2.003 Å

Chemicals

ChemComp-MN:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-MG:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-DTP:
2'-DEOXYADENOSINE 5'-TRIPHOSPHATE

ChemComp-HOH:
WATER

ChemComp-PGE:
TRIETHYLENE GLYCOL

Source
  • thermococcus kodakarensis (strain atcc baa-918 / jcm 12380 / kod1) (archaea)
  • synthetic construct (others)
  • thermococcus sp. (strain 9on-7) (archaea)
  • thermococcus sp. 9on-7 (archaea)
KeywordsDNA BINDING PROTEIN / DNA replication DNA polymerase archaea / TRANSFERASE / ternary complex / DNA polymerase / triphosphate

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