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Structure paper

TitleInsights into the structural and mechanistic basis of multifunctional S. cerevisiae Pif1p helicase.
Journal, issue, pagesNucleic Acids Res., Vol. 46, Page 1486-1500, Year 2018
Publish dateJun 6, 2017 (structure data deposition date)
AuthorsLu, K.Y. / Chen, W.F. / Rety, S. / Liu, N.N. / Wu, W.Q. / Dai, Y.X. / Li, D. / Ma, H.Y. / Dou, S.X. / Xi, X.G.
External linksNucleic Acids Res. / PubMed:29202194
MethodsX-ray diffraction
Resolution2.029 - 3.345 Å
Structure data

PDB-5o6b:
Structure of ScPif1 in complex with GGGTTTT and ADP-AlF4
Method: X-RAY DIFFRACTION / Resolution: 2.029 Å

PDB-5o6d:
Structure of ScPif1 in complex with polydT and ATPgS
Method: X-RAY DIFFRACTION / Resolution: 3.283 Å

PDB-5o6e:
Structure of ScPif1 in complex with TTTGGGTT and ADP-AlF4
Method: X-RAY DIFFRACTION / Resolution: 3.345 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-ALF:
TETRAFLUOROALUMINATE ION

ChemComp-MG:
Unknown entry

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

ChemComp-AGS:
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogue*YM

ChemComp-CL:
Unknown entry

Source
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae s288c (yeast)
KeywordsHYDROLASE / Helicase SF1 ssDNA ATP analog

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