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TitleEngineering a short-chain dehydrogenase/reductase for the stereoselective production of (2S,3R,4S)-4-hydroxyisoleucine with three asymmetric centers
Journal, issue, pagesSci Rep, Vol. 7, Page 13703-13703, Year 2017
Publish dateSep 13, 2016 (structure data deposition date)
AuthorsShi, X. / Miyakawa, T. / Nakamura, A. / Hou, F. / Hibi, M. / Ogawa, J. / Kwon, Y. / Tanokura, M.
External linksSci Rep / PubMed:29057974
MethodsX-ray diffraction
Resolution1.9 - 2.35 Å
Structure data

PDB-5gwr:
4-hydroxyisoleucine dehydrogenase complexed with NADH
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-5gws:
4-hydroxyisoleucine dehydrogenase complexed with NADH and succinate
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-5gwt:
4-hydroxyisoleucine dehydrogenase mutant complexed with NADH and succinate
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-SIN:
SUCCINIC ACID

Source
  • bacillus thuringiensis (bacteria)
KeywordsOXIDOREDUCTASE / NADH-dependent / short-chain / dehydrogenase / reductase

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