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Structure paper

TitleCombined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent.
Journal, issue, pagesMol. Cell, Vol. 68, Page 210-223.e6, Year 2017
Publish dateAug 28, 2017 (structure data deposition date)
AuthorsJost, M. / Chen, Y. / Gilbert, L.A. / Horlbeck, M.A. / Krenning, L. / Menchon, G. / Rai, A. / Cho, M.Y. / Stern, J.J. / Prota, A.E. ...Jost, M. / Chen, Y. / Gilbert, L.A. / Horlbeck, M.A. / Krenning, L. / Menchon, G. / Rai, A. / Cho, M.Y. / Stern, J.J. / Prota, A.E. / Kampmann, M. / Akhmanova, A. / Steinmetz, M.O. / Tanenbaum, M.E. / Weissman, J.S.
External linksMol. Cell / PubMed:28985505
MethodsX-ray diffraction
Resolution2.402 Å
Structure data

PDB-5ov7:
tubulin - rigosertib complex
Method: X-RAY DIFFRACTION / Resolution: 2.402 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-CA:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-6FS:
N-[2-methoxy-5-({[(E)-2-(2,4,6-trimethoxyphenyl)ethenyl]sulfonyl}methyl)phenyl]glycine

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-ACP:
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / AMP-PCP, energy-carrying molecule analogue*YM

ChemComp-HOH:
WATER

Source
  • rattus norvegicus (Norway rat)
  • gallus gallus (chicken)
  • bos taurus (domestic cattle)
KeywordsCELL CYCLE / TUBULIN FOLD / CYTOSKELETON / MICROTUBULE

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