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TitleSubstitution of Aromatic Residues with Polar Residues in the Active Site Pocket of epi-Isozizaene Synthase Leads to the Generation of New Cyclic Sesquiterpenes.
Journal, issue, pagesBiochemistry, Vol. 56, Page 5798-5811, Year 2017
Publish dateSep 6, 2017 (structure data deposition date)
AuthorsBlank, P.N. / Barrow, G.H. / Chou, W.K.W. / Duan, L. / Cane, D.E. / Christianson, D.W.
External linksBiochemistry / PubMed:28967743
MethodsX-ray diffraction
Resolution1.8 - 2.403 Å
Structure data

PDB-6ax9:
F95N Epi-isozizaene synthase
Method: X-RAY DIFFRACTION / Resolution: 2.403 Å

PDB-6axm:
F95Y Epi-isozizaene synthase
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-6axn:
F95C Epi-isozizaene synthase
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6axo:
F96S Epi-isozizaene synthase
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6axu:
W203Y Epi-isozizaene synthase
Method: X-RAY DIFFRACTION / Resolution: 1.822 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-POP:
PYROPHOSPHATE 2-

ChemComp-BTM:
N-benzyl-N,N-diethylethanaminium

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

Source
  • streptomyces coelicolor (bacteria)
  • streptomyces coelicolor (strain atcc baa-471 / a3(2) / m145) (bacteria)
KeywordsLYASE / Terpenoid Cyclase

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