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TitleCryoelectron Microscopy Reconstructions of the Pseudomonas aeruginosa and Neisseria gonorrhoeae Type IV Pili at Sub-nanometer Resolution.
Journal, issue, pagesStructure, Vol. 25, Issue 9, Page 1423-11435.e4, Year 2017
Publish dateSep 5, 2017
AuthorsFengbin Wang / Mathieu Coureuil / Tomasz Osinski / Albina Orlova / Tuba Altindal / Gaël Gesbert / Xavier Nassif / Edward H Egelman / Lisa Craig /
PubMed AbstractWe report here cryoelectron microscopy reconstructions of type IV pili (T4P) from two important human pathogens, Pseudomonas aeruginosa and Neisseria gonorrhoeae, at ∼ 8 and 5 Å resolution, ...We report here cryoelectron microscopy reconstructions of type IV pili (T4P) from two important human pathogens, Pseudomonas aeruginosa and Neisseria gonorrhoeae, at ∼ 8 and 5 Å resolution, respectively. The two structures reveal distinct arrangements of the pilin globular domains on the pilus surfaces, which impart different helical parameters, but similar packing of the conserved N-terminal α helices, α1, in the filament core. In contrast to the continuous α helix seen in the X-ray crystal structures of the P. aeruginosa and N. gonorrhoeae pilin subunits, α1 in the pilus filaments has a melted segment located between conserved helix-breaking residues Gly14 and Pro22, as seen for the Neisseria meningitidis T4P. Using mutagenesis we show that Pro22 is critical for pilus assembly, as are Thr2 and Glu5, which are positioned to interact in the hydrophobic filament core. These structures provide a framework for understanding T4P assembly, function, and biophysical properties.
External linksStructure / PubMed:28877506 / PubMed Central
MethodsEM (helical sym.)
Resolution5.1 - 8.0 Å
Structure data

8739

5vxx

EMDB-8739, PDB-5vxx:
Cryo-EM reconstruction of Neisseria gonorrhoeae Type IV pilus
Method: EM (helical sym.) / Resolution: 5.1 Å

8740

5vxy

EMDB-8740, PDB-5vxy:
Cryo-EM reconstruction of PAK pilus from Pseudomonas aeruginosa
Method: EM (helical sym.) / Resolution: 8.0 Å

Chemicals

ChemComp-OPE:
PHOSPHORIC ACID MONO-(2-AMINO-ETHYL) ESTER

Source
  • neisseria gonorrhoeae (bacteria)
  • pseudomonas aeruginosa pak (bacteria)
KeywordsPROTEIN FIBRIL / Melted helix / type IV pili

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