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TitleAmide linkages mimic phosphates in RNA interactions with proteins and are well tolerated in the guide strand of short interfering RNAs.
Journal, issue, pagesNucleic Acids Res., Vol. 45, Page 8142-8155, Year 2017
Publish dateMar 27, 2017 (structure data deposition date)
AuthorsMutisya, D. / Hardcastle, T. / Cheruiyot, S.K. / Pallan, P.S. / Kennedy, S.D. / Egli, M. / Kelley, M.L. / Smith, A.V.B. / Rozners, E.
External linksNucleic Acids Res. / PubMed:28854734
MethodsX-ray diffraction
Resolution1.95 Å
Structure data

PDB-5vaj:
BhRNase H - amide-RNA/DNA complex
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-MG:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

Source
  • bacillus halodurans (bacteria)
  • synthetic construct (others)
Keywordshydrolase/rna/dna / Amide-RNA / siRNA / RNA X-ray structure / phosphate modification / RIBONUCLEASE H / amide modified RNA-DNA. / hydrolase-rna-dna complex

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