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TitleCrystal structures of TdsC, a dibenzothiophene monooxygenase from the thermophile Paenibacillus sp. A11-2, reveal potential for expanding its substrate selectivity.
Journal, issue, pagesJ. Biol. Chem., Vol. 292, Page 15804-15813, Year 2017
Publish dateMar 16, 2017 (structure data deposition date)
AuthorsHino, T. / Hamamoto, H. / Suzuki, H. / Yagi, H. / Ohshiro, T. / Nagano, S.
External linksJ. Biol. Chem. / PubMed:28768765
MethodsX-ray diffraction
Resolution1.5785 - 1.9 Å
Structure data

PDB-5xb8:
Crystal structure of dibenzothiophene monooxygenase (TdsC) from Paenibacillus sp. A11-2
Method: X-RAY DIFFRACTION / Resolution: 1.795 Å

PDB-5xdb:
Crystal structure of FMN-bound TdsC from Paenibacillus sp. A11-2
Method: X-RAY DIFFRACTION / Resolution: 1.811 Å

PDB-5xdc:
Crystal structure of Indole-bound TdsC from Paenibacillus sp. A11-2
Method: X-RAY DIFFRACTION / Resolution: 1.5785 Å

PDB-5xdd:
Crystal structure of tertiary complex of TdsC from Paenibacillus sp. A11-2 with FMN and Indole
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5xde:
Crystal structure of tertiary complex of TdsC from Paenibacillus sp. A11-2 with FMN and dibenzothiophene
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-5xdg:
Crystal structure of tertiary complex of TdsC from Paenibacillus sp. A11-2 with FMN and dibenzothiophene sulfoxide
Method: X-RAY DIFFRACTION / Resolution: 1.747 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-GOL:
GLYCEROL

ChemComp-IND:
INDOLE

ChemComp-83R:
dibenzothiophene

ChemComp-83U:
dibenzothiophene 5-oxide

Source
  • paenibacillus sp. a11-2 (bacteria)
KeywordsOXIDOREDUCTASE / Monooxygenase / FMN binding protein / tetramer

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