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TitleStructural basis of TIR-domain-assembly formation in MAL- and MyD88-dependent TLR4 signaling.
Journal, issue, pagesNat Struct Mol Biol, Vol. 24, Issue 9, Page 743-751, Year 2017
Publish dateJul 31, 2017
AuthorsThomas Ve / Parimala R Vajjhala / Andrew Hedger / Tristan Croll / Frank DiMaio / Shane Horsefield / Xiong Yu / Peter Lavrencic / Zahid Hassan / Garry P Morgan / Ashley Mansell / Mehdi Mobli / Ailis O'Carroll / Brieuc Chauvin / Yann Gambin / Emma Sierecki / Michael J Landsberg / Katryn J Stacey / Edward H Egelman / Bostjan Kobe /
PubMed AbstractToll-like receptor (TLR) signaling is a key innate immunity response to pathogens. Recruitment of signaling adapters such as MAL (TIRAP) and MyD88 to the TLRs requires Toll/interleukin-1 receptor ...Toll-like receptor (TLR) signaling is a key innate immunity response to pathogens. Recruitment of signaling adapters such as MAL (TIRAP) and MyD88 to the TLRs requires Toll/interleukin-1 receptor (TIR)-domain interactions, which remain structurally elusive. Here we show that MAL TIR domains spontaneously and reversibly form filaments in vitro. They also form cofilaments with TLR4 TIR domains and induce formation of MyD88 assemblies. A 7-Å-resolution cryo-EM structure reveals a stable MAL protofilament consisting of two parallel strands of TIR-domain subunits in a BB-loop-mediated head-to-tail arrangement. Interface residues that are important for the interaction are conserved among different TIR domains. Although large filaments of TLR4, MAL or MyD88 are unlikely to form during cellular signaling, structure-guided mutagenesis, combined with in vivo interaction assays, demonstrated that the MAL interactions defined within the filament represent a template for a conserved mode of TIR-domain interaction involved in both TLR and interleukin-1 receptor signaling.
External linksNat Struct Mol Biol / PubMed:28759049 / PubMed Central
MethodsEM (helical sym.)
Resolution7.0 Å
Structure data

8625

5uzb

EMDB-8625, PDB-5uzb:
Cryo-EM structure of the MAL TIR domain filament
Method: EM (helical sym.) / Resolution: 7.0 Å

Source
  • homo sapiens (human)
KeywordsIMMUNE SYSTEM / TIR domain / adaptor proteins / TLR signaling / homotypic protein interactions

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