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Structure paper

TitleTime-Dependent Extension from an 8-Oxoguanine Lesion by Human DNA Polymerase Beta.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 139, Page 9684-9690, Year 2017
Publish dateMay 11, 2017 (structure data deposition date)
AuthorsReed, A.J. / Suo, Z.
External linksJ. Am. Chem. Soc. / PubMed:28682600
MethodsX-ray diffraction
Resolution1.7 - 2.58 Å
Structure data

PDB-5vrw:
Human DNA polymerase beta pre-catalytic 8-oxoG:dC extension complex with dTTP bound in non-planar conformation
Method: X-RAY DIFFRACTION / Resolution: 2.58 Å

PDB-5vrx:
Human DNA polymerase beta pre-catalytic 8-oxoG:dC extension complex with dTTP bound in Watson-Crick conformation
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-5vry:
Human DNA polymerase beta 8-oxoG:dC extension with dTTP after 20 s
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5vrz:
Human DNA polymerase beta 8-oxoG:dC extension with dTTP after 60 s
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-5vs0:
Human DNA polymerase beta 8-oxoG:dC extension with dTTP after 80 s
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-5vs1:
Human DNA polymerase beta pre-catalytic 8-oxoG:dA extension complex with dTTP bound in non-planar conformation
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5vs2:
Human DNA polymerase beta pre-catalytic 8-oxoG:dA extension complex with dTTP bound in Watson-Crick conformation
Method: X-RAY DIFFRACTION / Resolution: 2.33 Å

PDB-5vs3:
Human DNA polymerase beta 8-oxoG:dA extension with dTTP after 90 s
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-5vs4:
Human DNA polymerase beta 8-oxoG:dA extension with dTTP after 120 s
Method: X-RAY DIFFRACTION / Resolution: 1.87 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-TTP:
THYMIDINE-5'-TRIPHOSPHATE

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

ChemComp-IMD:
IMIDAZOLE

ChemComp-ACT:
ACETATE ION

ChemComp-MG:
Unknown entry

ChemComp-PPV:
PYROPHOSPHATE

ChemComp-CL:
Unknown entry

ChemComp-NA:
Unknown entry

Source
  • homo sapiens (human)
  • synthetic construct (others)
Keywordstransferase / lyase/dna / polymerase / 8-oxoguanine / BER / lyase-dna complex

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