+検索条件
-Structure paper
タイトル | Addressing preferred specimen orientation in single-particle cryo-EM through tilting. |
---|---|
ジャーナル・号・ページ | Nat Methods, Vol. 14, Issue 8, Page 793-796, Year 2017 |
掲載日 | 2017年7月3日 |
著者 | Yong Zi Tan / Philip R Baldwin / Joseph H Davis / James R Williamson / Clinton S Potter / Bridget Carragher / Dmitry Lyumkis / |
PubMed 要旨 | We present a strategy for tackling preferred specimen orientation in single-particle cryogenic electron microscopy by employing tilts during data collection. We also describe a tool to quantify the ...We present a strategy for tackling preferred specimen orientation in single-particle cryogenic electron microscopy by employing tilts during data collection. We also describe a tool to quantify the resulting directional resolution using 3D Fourier shell correlation volumes. We applied these methods to determine the structures at near-atomic resolution of the influenza hemagglutinin trimer, which adopts a highly preferred specimen orientation, and of ribosomal biogenesis intermediates, which adopt moderately preferred orientations. |
リンク | Nat Methods / PubMed:28671674 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 4.2 Å |
構造データ | EMDB-8731: |
由来 |
|