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TitleBiochemical Characterization and Structural Basis of Reactivity and Regioselectivity Differences between Burkholderia thailandensis and Burkholderia glumae 1,6-Didesmethyltoxoflavin N-Methyltransferase.
Journal, issue, pagesBiochemistry, Vol. 56, Page 3934-3944, Year 2017
Publish dateJan 5, 2017 (structure data deposition date)
AuthorsFenwick, M.K. / Almabruk, K.H. / Ealick, S.E. / Begley, T.P. / Philmus, B.
External linksBiochemistry / PubMed:28665591
MethodsX-ray diffraction
Resolution1.39 - 1.77 Å
Structure data

PDB-5ufm:
Crystal structure of Burkholderia thailandensis 1,6-didemethyltoxoflavin-N1-methyltransferase with bound 1,6-didemethyltoxoflavin and S-adenosylhomocysteine
Method: X-RAY DIFFRACTION / Resolution: 1.77 Å

PDB-5ufn:
Crystal structure of Burkholderia thailandensis 1,6-didemethyltoxoflavin-N1-methyltransferase with bound S-adenosylhomocysteine
Method: X-RAY DIFFRACTION / Resolution: 1.39 Å

Chemicals

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-AZ8:
pyrimido[5,4-e][1,2,4]triazine-5,7(6H,8H)-dione

ChemComp-SO4:
SULFATE ION

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-HOH:
WATER

Source
  • burkholderia thailandensis (strain atcc 700388 / dsm 13276 / cip 106301 / e264) (bacteria)
KeywordsTRANSFERASE / N-methyltransferase / S-adenosylmethionine-dependent / Complex

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