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Structure paper

TitleEfficient switching of mCherry fluorescence using chemical caging.
Journal, issue, pagesProc. Natl. Acad. Sci. U.S.A., Vol. 114, Page 7013-7018, Year 2017
Publish dateDec 22, 2015 (structure data deposition date)
AuthorsCloin, B.M.C. / De Zitter, E. / Salas, D. / Gielen, V. / Folkers, G.E. / Mikhaylova, M. / Bergeler, M. / Krajnik, B. / Harvey, J. / Hoogenraad, C.C. ...Cloin, B.M.C. / De Zitter, E. / Salas, D. / Gielen, V. / Folkers, G.E. / Mikhaylova, M. / Bergeler, M. / Krajnik, B. / Harvey, J. / Hoogenraad, C.C. / Van Meervelt, L. / Dedecker, P. / Kapitein, L.C.
External linksProc. Natl. Acad. Sci. U.S.A. / PubMed:28630286
MethodsX-ray diffraction
Resolution1.55 Å
Structure data

PDB-5fhv:
Crystal structure of mCherry after reaction with 2-mercaptoethanol
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

Chemicals

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-BME:
BETA-MERCAPTOETHANOL

ChemComp-P6G:
HEXAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

Source
  • discosoma sp. (sea anemone)
KeywordsFLUORESCENT PROTEIN / 2-mercaptoethanol / Michael addition

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