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Structure paper

TitleAntibacterial Nucleoside-Analog Inhibitor of Bacterial RNA Polymerase.
Journal, issue, pagesCell, Vol. 169, Page 1240-1248.e23, Year 2017
Publish dateJan 29, 2017 (structure data deposition date)
AuthorsMaffioli, S.I. / Zhang, Y. / Degen, D. / Carzaniga, T. / Del Gatto, G. / Serina, S. / Monciardini, P. / Mazzetti, C. / Guglierame, P. / Candiani, G. ...Maffioli, S.I. / Zhang, Y. / Degen, D. / Carzaniga, T. / Del Gatto, G. / Serina, S. / Monciardini, P. / Mazzetti, C. / Guglierame, P. / Candiani, G. / Chiriac, A.I. / Facchetti, G. / Kaltofen, P. / Sahl, H.G. / Deho, G. / Donadio, S. / Ebright, R.H.
External linksCell / PubMed:28622509
MethodsX-ray diffraction
Resolution3.323 - 3.35 Å
Structure data

PDB-5x21:
Crystal structure of Thermus thermophilus transcription initiation complex with GpA and pseudouridimycin (PUM)
Method: X-RAY DIFFRACTION / Resolution: 3.323 Å

PDB-5x22:
Crystal structure of Thermus thermophilus transcription initiation complex with GpA and CMPcPP
Method: X-RAY DIFFRACTION / Resolution: 3.35 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-PUM:
(1S)-1,4-anhydro-5-[(N-carbamimidoylglycyl-N~2~-hydroxy-L-glutaminyl)amino]-5-deoxy-1-(2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-D-ribitol / antibiotic*YM

ChemComp-HOH:
WATER

ChemComp-2TM:
5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine

Source
  • thermus thermophilus (bacteria)
  • thermus thermophilus (strain hb27 / atcc baa-163 / dsm 7039) (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
KeywordsTRANSFERASE/DNA / Transcription / RNA polymerase / transcription inhibition / nucleoside-analog inhibitor / pseudouridimycin / TRANSFERASE-DNA complex / primer-dependent initiation

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