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Structure paper

TitleStructural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1
Journal, issue, pagesMol. Cell, Vol. 67, Page 139-147.e2, Year 2017
Publish dateApr 19, 2017 (structure data deposition date)
AuthorsNishimasu, H. / Yamano, T. / Gao, L. / Zhang, F. / Ishitani, R. / Nureki, O.
External linksMol. Cell / PubMed:28595896
MethodsX-ray diffraction
Resolution2 Å
Structure data

PDB-5xh6:
Crystal structure of the Acidaminococcus sp. BV3L6 Cpf1 RVR variant in complex with crRNA and target DNA (TATA PAM)
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-5xh7:
Crystal structure of the Acidaminococcus sp. BV3L6 Cpf1 RR variant in complex with crRNA and target DNA (TCCA PAM)
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

Source
  • acidaminococcus sp. (strain bv3l6) (bacteria)
  • acidaminococcus sp. bv3l6 (bacteria)
KeywordsHYDROLASE/RNA/DNA / nuclease / HYDROLASE-RNA-DNA complex

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