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Structure paper

TitleStructural Basis for Expansion of the Genetic Alphabet with an Artificial Nucleobase Pair.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 56, Page 12000-12003, Year 2017
Publish dateMar 31, 2017 (structure data deposition date)
AuthorsBetz, K. / Kimoto, M. / Diederichs, K. / Hirao, I. / Marx, A.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:28594080
MethodsX-ray diffraction
Resolution1.7 Å
Structure data

PDB-5nkl:
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in a closed ternary complex with the artificial base pair dDs-dPxTP
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-91T:
[[(2~{R},3~{S},5~{R})-5-[4-[(4~{R})-4,5-bis(oxidanyl)pent-1-ynyl]-2-nitro-pyrrol-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate

ChemComp-91R:
[[(2~{R},3~{S},5~{R})-5-[4-[(4~{S})-4,5-bis(oxidanyl)pent-1-ynyl]-2-nitro-pyrrol-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • thermus aquaticus (bacteria)
  • synthetic construct (others)
KeywordsTRANSFERASE / DNA polymerase / artificial base pair / unnatural base pair / ternary complex

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